At this time of the year, at this holidays season we are surrounded by cookies, cakes, candies and other sweeties that not really good for our digestive system, especially for people with gluten sensitivity and gluten intolerance, it may be downright nightmare.

The most common treatment for gluten intolerance is avoiding food containing gluten; those are wheat, rye, oat, spelt and all pasta, cookies, cakes made with those grains.

Avoiding gluten is quite challenging. It is not just giving up pasta, crackers, cookies (except for Chelsea Clinton's wedding cake). Gluten is common food additive, often used in small amounts to increase protein content, it may be found even in ketchup or ice-cream.

People with gluten sensitivity and gluten intolerance do not have specific enzymes needed for gluten digestion, it may be congenital-they were born with this condition, in this case it called celiac sprue, it is chronic inflammation in the small intestine, or acquired -due to history of intestinal infection or yeast overgrowth that sets stage for chronic inflammation in the small intestine too, producing leaky gut syndrome and food allergy.

Digestive enzymes supplements may help, but it should be specific combination of enzymes in this case, they should withstand acidic environment of stomach: hydrochloric acid and pepsin produced by stomach mucosa.

Digestion of gluten, casein (protein in dairy), soy require presence of some peptidase and protease- protein digesting enzymes. BioCore DPP IV from National Enzyme Company is combination of these enzymes helping in gluten digestion. In nature, gluten proteins are attached to starches-carbohydrates. For complete gluten breakdown, amylase is required in addition to the proteases. The goal is to break up the large gluten molecules into smaller pieces before they leave the stomach.

Digestive enzymes have been used very successfully for gluten intolerance, allowing some patients to completely eliminate gluten-free diet. Others use enzymes only occasionally when they eat food that contains gluten. Both celiac and non-celiac patients find that adding enzymes improves the quality of their life.

A lot of patients with gluten sensitivity have problem with casein and lactose digestion, that is why it is so important to take good quality combination of digestive enzymes for gluten, casein digestion accompanied by amylase (for carbohydrate digestion), lipase (for fats digestion), lactase (for lactose digestion)- broad spectrum of digestive enzymes.

There is scientific data, indicating that following gluten-free diet for long period of time is not good, population of good, beneficial intestinal bacteria such as Bifidobacterium and Lactobacillus decreased, while bad bacteria-Escherichia coli increased. This condition will compromise intestinal health and immune system, which makes patients more susceptible for infections and cancer development.

Therefore, managing gluten intolerance with supplemental enzymes would be preferable to a completely gluten-free diet, which will reduce beneficial intestinal microflora.

According to study from the University of Maryland's Center for Celiac Research, the incidence of celiac disease doubles every 15 years, and now we know that celiac disease may be developed later in life. The reasons for gluten intolerance emerging later in life are currently unclear. The amount of ingested gluten, the condition of the intestinal microflora and its change over time, intestinal infections and medications causing yeast overgrowth-all these factors effect condition of small intestine and immune response.

In our office we use Standard Process supplements, we use Multizyme, containing digestive enzymes and pancreatin needed for proper breakdown and absorption of proteins, carbohydrates, sugars and fats. We also use with great success Zymex -for maintaining of healthy intestinal flora and ZymexII, containing papain, bromelain-for protein digestion, amylase, cellulose -for carbohydrate digestion and lipase– for fats digestion. All patients with gluten sensitivity and gluten intolerance should take good quality Probiotic ( friendly bacteria such as Bifidobacterium, Lactobacillus) and Prebiotic Inulin- to maintain good condition for beneficial flora in the intestine.

About the author:

Source: http://www.sooperarticles.com/health-fitness-articles/diseases-articles/gluten-sensitivity-gluten-intolerance-269201.html


lipase

36 thoughts on “Lipase

  1. Ivy

    How to prove Orlistat is an inhibitor for lipase?
    I need to design an experiment to prove that Orlistat is an inhibitor of lipase. Do you have any suggestion on how i might start?

    1. misoma5

      Well, if it’s an in vittro experiment , get some lipase (sold as pancreatic enzyme) and place lipase in 2 test tubes. Add orlistat to one and add lipids to both shake and see the results.

      If it is an in vivo experiment, get several of your friends to eat a high fat meal. Give half orlistat. See the results….you’d expect smelly,fatty stools that float in the group that took orlistat and normal stools in those that didn’t.

  2. KCWS

    What tests can be used to find out if a substance contains the enzymes LIPASE or PROTEASE?
    Are there any tests that can be used to find out if a substance contains the enzymes LIPASE or PROTEASE? And are there other tests that can be used to find out if a substance contains other enzymes?
    If so, please list and explain.
    Thanks in advance!

    1. Simonizer1218

      The only way to test for lipase and or protease is to assay for those enzymes DIRECTLY. Using a biuret test will only tell you if a peptide because it detects peptide bonds. So, it will be positive for pretty much evey enzyme, not just lipase and protease. To detect a particular enzyme, you must use a test selective for THAT enzyme, and then maybe repeat the test with a known inhibitor of that enzyme.

  3. feline*love

    Whats the difference between lipase and trigylcerides?
    Are the two related or completely different things? My lipase is normal and my trigs is 333. Can a nurse explain that to me or even a med student thanks.
    I am diabetic. my Ac1 level is 8.9

  4. mary p

    how dangerous is it to live with a lipase deficiency?
    What can it lead to if untreated (diabetes, cancer, etc) and how do you find out if you have a lipase deficiency?

    1. Pharmjust

      Lipase is needed to break down fats from your diet to produce components for cell membranes. If you had a severe lipase deficiency, you would be dead.

      How do you know that you have a lipase deficiency? The effect depends on which lipase is deficient.

  5. Blank

    What would cause an elevated Lipase blood test, but normal CT scan of pancreas?
    I have an elevated Lipase test for pancreatitis, but my CT scan results were normal of the pancreas. What would cause elevated lipase? What diseases cause elevated lipase?

    1. Spreedog

      “Peaches” is correct. When a doctor orders a test, it is the responsibility of that doctor to explain the results. An elevated lipase must be evaluated in the context of your medical history, physical examination, and many other parameters such as your blood tests for renal (kidney) function, liver function, the complete blood count (CBC), and usually a serum amylase which is often done with the serum lipase. We need all of this information – which your doctor has.

      If you have obtained your own lab results – fine, but make sure you discuss these results when you see you physician – – and make sure you pay attention. I always recommend having a friend or family member along when I explained these things to patients. An extra set of ears helps you recall what is being explained. Sometimes doctors actually do spend time explaining things carefully, and the patients have trouble hearing or understanding what is said.

      Caution – It’s not a good idea to place an emergency after hours call to a doctor late at night to explain a lab result – especially if they have explained it already. Even the most caring and patient doctors get a bit upset about getting these calls late at night. Doctors are not perfect – we get tired and grumpy too. This website is not a bad place for this question after hours. ‘ Quite a few medical people on this site. I expect you will get your answer at your next appointment.

      The negative CT scan only helps exclude causes such as a pancreatic cancer causing this lipase elevation, but that should be comforting news. Whatever the cause (and there are many possibilities), it is less likely to be a malignancy with a negative CT scan. Remember that CT scans do not “see” everything. There is no perfect test in medicine.
      CT scans usually cannot “see” or diagnose pancreatitis.

  6. Terri S

    Does taking the Alli diet pill effect your pancreatic lipase levels?
    About a month ago, I had some severe stomach pains. The pain would then redirect to my back – some of the worst pain I’ve ever encountered. I went to the doctor and got some blood work done and they said that my pancreatic lipase levels were high. Just two weeks before, I had stopped taking Alli and I know Alli inhibits lipase enzymes. I was just wondering if anyone can verify if there is a connection between the two.

  7. Mark

    Can high concentrations of bile salts inhibit pancreatic lipase activity?
    I know that, at certain concentrations, bile salts increase the activity of lipase but i read that at concentrations which are too high; the lipase is inhibited.

    Is this true?

    1. Peter S

      Yes -but because of colipase the lipases work,see below.

      Lipases are inactive on an emulsified triglyceride substrate in the presence of micellar concentrations of bile salts, such as those found in the small intestine during digestion. Bile salts are amphiphilic molecules mainly found adsorbed at the oil-water interface or dispersed as mixed micelles in solution. The bile salt coating of triglyceride globules prevents the pancreatic lipase adsorption, and thus lipolysis, at the oil-water interface. However, colipase, a specific lipase-anchoring protein present in the exocrine secretion of pancreas, counteracts this effect through the formation of a specific 1:1 complex with lipase that facilitates its adsorption at bile salt-covered lipid-water interface.

  8. Mark

    Can high concentrations of bile salts inhibit pancreatic lipase activity?
    I know that, at certain concentrations, bile salts increase the activity of lipase but i read that at concentrations which are too high; the lipase is inhibited.

    Is this true?

    1. Peter S

      Yes -but because of colipase the lipases work,see below.

      Lipases are inactive on an emulsified triglyceride substrate in the presence of micellar concentrations of bile salts, such as those found in the small intestine during digestion. Bile salts are amphiphilic molecules mainly found adsorbed at the oil-water interface or dispersed as mixed micelles in solution. The bile salt coating of triglyceride globules prevents the pancreatic lipase adsorption, and thus lipolysis, at the oil-water interface. However, colipase, a specific lipase-anchoring protein present in the exocrine secretion of pancreas, counteracts this effect through the formation of a specific 1:1 complex with lipase that facilitates its adsorption at bile salt-covered lipid-water interface.

  9. Surya

    what is the medicine to produce the enzymes proteases & peptidases and lipases?
    producing of the enzymes proteases & peptidases (for digestion of proteins) and lipases (for digestion of fats).are hampered and causing indigestion so what medicine should i take to produce
    proteases & peptidases and lipases ?

    1. dr_anup

      Enzycip AQ – it is the medicine which contains all the digestive enzymes including proteases,peptidase and lipases. It also contains carbohydrate digesting enzymes-cellulase and amylase as well. A product of renowned company Cipla pharmaceuticals

  10. dagg

    What is the effect of temperature on lipase?
    What is the optimum temperature for lipase to work at?

    For example, when I add lipase to milk with sodium carbonate at which temperature would the reaction occur most effectively at?

    1. eli

      normal body temp is 98.6
      too much variation from that in either direction will make most proteins denature
      lipase is an enzyme
      enzymes are made of proteins

  11. Sarah :)

    why does lipase breaking down fat lower the pH?
    We did an experiment and after time the pH got lower as lipase broke down more of the fat in milk to fatty acid and glycerol. I understand what happens but I don’t know why the pH becomes more acidic (well it goes from 8.6-7.4) and there is bile and sodium carbonate in the solution too which is what makes it alkaline

    1. Mike T

      when the lipase breaks down the fat molecules which releases H+ ions which essentially makes the solution more acidic since pH really means -log[H+] so as the H+ concentration increases the pH decreases (more acidic). Hope this helps

  12. kariwithak0905

    What are some causes of high lipase enzymes?
    My niece is insulin dependant and her lipase enzymes are over
    700. She is on the pump and her sugars have been up and down for two weeks. She spent one week in the hospital when her sugars went up to 500. Any suggestions?

    1. drinda_house

      almost ANY abdominal pain (regardless of the cause) can raise the lipase level although VERY HIGH level points toward Pancreatitis (not likely in your cousin’s case).
      i think poorly controlled diabetes is another cause of high lipase level.
      SO ITS MORE OR LESS NON-SPECIFIC MARKER UNLESS ITS VERY HIGH & FIT THE PICTUR OF PANCREATITIS.

  13. Raven

    What form of glycerol is broken down by lipases?
    Glycerol D? or L? is broken down by lipases and then put through glycolysis and the rest of the metabolic process….
    Can’t seem to google this answer for myself. Thanks in advance for any help.

    1. Merlin's Feline

      glycerol is NOT broken down by lipases they work on triglycerides.The glycerol can be phosphorylated by glycerol kinase to glycerol phosphate thence to glyceraldehye 3-phosphate or to DHAP for glycolysis to use. Glycerol is not optically active as it is symmetrical ( no D or L )

  14. waudet2000

    If you have very little lipase in your blood could that contribute in inability to lose wt?
    Lipase helps your body to burn fat. I don’t know how much influence it would have but I would like to know. Thanks.

  15. dearvenice

    Do all mushrooms have the enzyme lipase?
    I need to know if all mushroom species have the enzyme lipase. I have this school report that involves mushrooms and lipase. To make the report successful, I need to prove that all mushroom species have lipase. Please answer me? I’d really appreciate it. Thanks!

    1. Marilyn

      First: replace the common name “mushroom” with a scientific name. Depending on your authority it may be 1) Basidiomycetes; 2) Ascomycetes & Basidiomycetes; or 3) some other definition.

      Second: understand Lipase: The title of link #1 is “What Makes A Fungus Virulent? It’s Lipase”.
      Link #2 Lipase is an enzyme …

      Third: understand the word “all”. This is a black vs white question. If any mushroom lives without lipase, then it does not matter how many depend on it for life. What organisms do not have lipids in their membranes? “Search” Viral Lipase and you will get lots of “hits”.

      Suspect the proof goes something like this: all fungi depend on cell membranes for life. Cell membranes contain lipids. Therefore, all fungi (not just mushrooms) must possess lipase.

  16. Hann

    Why is lipase elevated when no pancreatic disease?
    I have had thorough testing for an elevated lipase level. Everything has been normal and I have no symptoms. Are there other reasons for lipase elevation?

  17. stclair2012

    What are the substrates of Lactase, Maltase, Protease, and Lipase?
    What are the substrates of Lactase, Maltase, Protease, and Lipase?
    For example: The substrate of salivase is starch. Please help! -Thanks-

  18. HelloKitty

    investigating the effects of bile salts and lipase on the digestion of triglycerides in milk?
    why are lipase and water added to the milk? i know that the lipase contains enzymes which help digest the triglycerides into fatty acids but im not sure why the water is added? does anybody know? it it just a sort of control thing or to replicate the environment inside the stomach or something else? i really don’t know… if anybody could help me i would appreciate it so much xxx

  19. Ikuto Luv

    Is lipase an enzyme that can break down hydrocarbons?
    Is lipase an enzyme that can break down hydrocarbons?
    if not, what is an enzyme than can – one that I can possibly buy for a cheap price?
    10 POINTS!
    i mean the hydrocarbons themselves into carbons and hydrogens instead of hydrocarbons.

  20. badbluebabes

    What reaction is catalyzed by Lipase enzyme?
    I need help! I am working on a chem proj. where i hv to determine how the enzyme lipase catalyzes a particular reaction. I want to know which reactions a lipase can catalyze and what is the outcome of the reaction? –> meaning what happens in the reaction? so, if the reactions produces a particular change in color etc.
    plz be as quick as possible! will be GREAT help!!!!!!!!!!!!! n provide sources if u can :))))))))

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