Toll-like receptor 9 (TLR9) often known as CD289 (cluster of differentiation 289), is a member of the Toll-like receptor family that recognizes pathogen-associated molecular pattern. TLR9 was first cloned and identified as a receptor for unmethylated CpG-DNA as well as for bacterial DNA. It is essential not only for pro-inflammatory cytokine production and other inflammatory responses, but it also plays a role in the induction of T helper 1 (Th1) acquired immune response and in the proliferation of B cells. Like all other members of the TLR family, TLR9 is composed of an extracellular domain containing multiple leucine-rich repeats (LRRs), a transmembrane region, and a cytoplasmic tail containing the conserved TIR domain. The TLR9 sequence encodes a 1032 aa protein containing 27 N-terminal LRRs with a calculated molecular weight of 116 kDa . The gene for TLR9 has been mapped to human chromosome 3p21.3. TLR9 is most closely related to TLR7 and TLR8 with 36% and 35% overall amino acid sequence identity, respectively and thus along with TLR7 and TLR8 constitutes a new sub-family of the TLRs.

19 thoughts on “Negative Lupus Tests

  1. dumbbrunnett88

    What blood tests can be used to diagnose Lupus?
    I am almost positive that I have lupus, as I have almost every one of the symptoms. My doctor also thinks that i may have lupus, but every time i get a blood test, everything is negative. What tests can be used to diagnose lupus? Because i want to find out for sure if this is what I have
    I was already diagnosed with fibromylagia but my symptoms go beyond just that. I have also been tested numerous times for Lyme disease and other rheumatic diseases, all coming out negative.

    1. eabena

      Raised ESR. Reduced white cells, reduced lymphocytes, and/or reduced platelets. Serum antinuclear antibodies (ANA) is positive in almost all cases. Double-stranded DNA (dsDNA) is specific to lupus but is only present in 50% cases. Anti-Ro and anti-La can also be detected. 25% patients have rheumatoid factor. Reduced serum complement levels during active disease.

  2. JC

    I was just diagnosed with Alopecia Areata is there A chance I might Get Lupus?
    I know the two are related to autoimmune disease, and I tested negative for Lupus but is there a chance I can still develop Lupus? or does it not work that way?

    1. Cats

      No not necessarily at all. They are two different autoimmune disorders. Alopecia is pretty treatable and curable so don’t worry. They will give you a steroid cream and if that doesn’t work, injections.

      Good luck:)

  3. JC

    I was just diagnosed with Alopecia Areata is there A chance I might Get Lupus?
    I know the two are related to autoimmune disease, and I tested negative for Lupus but is there a chance I can still develop Lupus? or does it not work that way?

  4. mommie09

    what do you call a person who says that have different types of diseases to get attention?
    My mother says she has lupus and she tested negative for it, but she still tells people that she has it. she also says she has fibromyalgia, macular degeneration, and a ton of other diseases. and when doctors try to give her medicine for it she says that she is allergic. I don’t feel bad for her anymore when she tell me she is not feeling good. Am I a bad daughter for thinking that?

    1. Survivor

      Hypochondriac or I think Munchaesen Syndrome?? For some people that is the only way they know how to get some attention, just like bad kids, sometimes they are acting out purely for attention. Try paying special attention to her, don’t feed into the lies, but ask how she’s feeling or something and see if that helps the situation. You only have one mother, so do your best to help her when you can:)

  5. feroscious

    What could be the case of hip and joint paint to the point of not being able to sit or stand in a 10 yr old?
    He’s been sick for 4 months now and on the waiting for UCLA Children’s Hospital. All the blood tests are coming back normal as well as the MRI. The only thing he tested positive for was the rheumatoid factor (and slightly anemic) but he doesn’t have rheumatoid arthritis per 2 doctors. He’s starting to lose his balance when he stands/walks and can’t sit down for any amount of time without pain from the hips down to the ankles (including the muscles). The only comfortable position he can be in with the least amount of pain is flat on the floor – even sleeping on an extremely comfortable mattress hurts. Regardless of where he sleeps at night, he is woken up in the middle of the night with pain. He is taking naprosyn per the UCLA doctor until he can get in to see her. Has anyone else experienced this or know anything about this? He was negative for lupus, mono, lyme disease, ankylosing spondylitis.
    We are on the waiting list for a pediatric neurologist and pediatric ophthalmologist also. Watching my son in pain and being stuck in limbo because of the “system” is so frustrating. If it were their children they would see him. Our family doctor keeps calling to emphasize the urgency but it’s gotten us nowhere so far.

    1. Bliss J

      Is it possible he’s suffering from a form of dysplasia or fibromyalgia? Cushing’s Disease shares some of the symptoms as well, but that’s highly doubtful. The only other thing that crosses my mind would be MS or MD, but I’m sure he’s been tested for that already.

      On a brighter note, I do recommend looking into a SelectComfort Sleep Number bed for his comfort! SelectComfort provides units for both the Ronald McDonald House as well as for patients with cerebral palsy in order to aid in their patients’ comfort while resting/sleeping–they’re medically proven to be the only bed that measurably helps patients rest.

      I hope this helps a little–and you and the boy will be in our prayers…

  6. luffjm34

    What autoimmune disorder would cause these symptoms for so many years?
    -Extreme Fatigue
    -Never feel rested after sleep
    -Stomach problems (gas, cramps)
    -Low body temperature
    -raised SED rate/C Reactive protein

    I have tested negative for lupus, rheumatoid arthritis, Celiac’s and my thryoid number was a little off, but not out of the lab’s normal range. These symptoms have been for EIGHT YEARS.

    1. Anonymous

      Are you on a gluten-free diet? If not, consider doing this, and after 3 or so months, get all of your blood levels tested again. Celiac tests are notorious for providing false negatives, and the symptoms of Celiac disease can disguise themselves as a number of things, including cancer.

      I tested negative as a Celiac, but after the 1st month on a strict gluten-free diet, I noticed huge changes in my state of health. It was like I had a new lease on life. By 3 months, my thyroid was normal again and I have so much more energy.

      Good luck on your quest for health.

  7. Battlebatty

    How can I tell the difference between Fifth disease and Lupus?
    I have red blotches on my face, some minor rashes and i had a few ulcers last week, I just recently developed the blotches.
    My parents think I might have lupus.
    I have never been so scared in my life.

    1. SleepyRadish

      Fifth disease is something children get. Classic Lupus symptoms are: butterfly rash (redness on cheeks and across bridge of nose), sores in the nose or mouth, fatigue, joint pain, and dry mouth. Go see your family doctor. He can initiate some of the testing. A positive ANA (blood test) with a negative RA (blood test to show arthritic inflammation) would indicate Lupus. If he thinks you have it, he can refer you to a Rheumatologist. Please don’t be afraid. I was diagnosed with Lupus over 18 years ago. I take daily medication that keeps things under control.
      Anyway, it might be something else! Go get checked out, so you can quit worrying. Best of luck to you.

  8. ProactiveMom

    Is it hard to diagnose Lupus in a teen with no positive labs?
    There’s a strong family history and symptoms (including butterfly rash) that have lasted almost 4 months. Rheumy says he ruled out Lupus.

  9. Liz

    What physical symptoms can flea bites cause?
    Ive been having knee/leg pain also arm pain. Basically joint pain. I seen a rhematologist today and my doctor took arthritis tests, lupus, and some others, all negative. He noticed flea bites that i have from about two months ago. Their everywhere! Tons! He said that infections from other people or animals can cause these symptoms, and that they go away in about 6 weeks. Has anyone else ever had this? Or heard about it? This is new to me. I hope that’s all it is. I don’t want arthritis!


  10. lil miss girl

    What medical conditions have the same symptoms as fibromalgia?
    A certain number of tender points are required to diagnose fibromyalgia, but I was only sensitive at about half of them. I have most of the other symptoms associated with fibromyalgia, so, if that’s not what I have, what is my disease or condition? (Also tested negative for lupus.)

    1. zurama

      Conditions That Commonly Occur in Fibromyalgia Patients

      A number of conditions overlap or often co-exist with fibromyalgia that have similar symptoms. It is not clear if these conditions or others are risk factors for fibromyalgia, are direct causes, have common causes, or have no relationship at all with CFS.

      Chronic Fatigue Syndrome. There is a significant overlap between fibromyalgia and chronic fatigue syndrome (CFS). In a 2003 study, for example, 43% of CFS patients also were diagnosed with fibromyalgia. As with fibromyalgia, the cause of CFS is unknown and its course is chronic. Both disorders can be diagnosed by a physician only on the basis of symptoms reported by the patient and cannot be confirmed by laboratory tests or other objective measures. The two disorders share most of the same symptoms. They are even treated almost identically. The differences are primarily the following:

      * Fatigue is the dominant symptom in CFS. It is severe and not relieved by rest or sleep and not the result of excessive work or exercise.
      * Pain with tender points is the primary symptom in fibromyalgia. (Some patients with CFS exhibit similar tender pressure points. However, muscle pain is less prominent in patients with CFS.)

      Some physicians believe that fibromyalgia is simply an extreme variant of chronic fatigue syndrome. There is some physical evidence, however, that the two disorders are distinct, with treatments that are specific to each.

      Myofascial Pain Syndrome. Myofascial pain syndrome can be confused with fibromyalgia and may also accompany it. Unlike fibromyalgia, myofascial pain tends to occur in trigger points, as opposed to tender points, and typically there is no widespread, generalized pain. Trigger-point pain occurs in taut muscles, and when the doctor presses on these points, the patient may experience a muscle twitch. And unlike tender points, trigger points are often small lumps, about the size of a pencil eraser.

      Major Depression. The link between psychological disorders and fibromyalgia is very strong and problematic. Certain studies report that between 50% and 70% of fibromyalgia patients have a lifetime history of depression. Only between 18% and 36% of fibromyalgia patients, however, have concurrent major depression, a severe form of depression. It should be noted that some researchers have observed that people who have both psychological disorders and fibromyalgia are more likely to seek medical help than patients who simply have symptoms of fibromyalgia. Such findings may bias study results and favor a higher-than-actual association between depression and fibromyalgia.

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